Each datum is the result of three replicates that showed the same result , revived; , not revived; NE, not examined aViability of stock cultures preserved for 60 months by the sawdust-freezing method was determined after alternate freezing and thawing at 6month intervals bViability of stock cultures for 120 months by the sawdust-freezing method was determined for stocks kept in a freezer without alternate freezing and thawing treatment