Metallic silver in the form of SNPs has made a remarkable come-back as a potential antimicrobial as well as antiviral agent (Gaikwadet al., 2013b). As the several pathogenic bacteria have develop-ing resistance against various antibiotics, the SNPs could be usedagainst such bacteria. SNPs have been studied and applied in manyareas including biomedical, agricultural, electronic fields, and tex-tile, etc. Researchers have been working on new, eco-friendly andcheaper methods to make products of colloidal silver. Due to theease of biological method, metal nanoparticles have been studiedby many researchers that produce small particles stabilized by pro-tein. The mechanism involved in this production has not yet beenrevealed, however hypothetical mechanisms have been proposedin the literature. The present study has been focused on synthesisof SNPs by P. glomerata. The effect of physical factors, salt concen-tration and filtrate amount was also evaluated.