Further,artificial insemination performed in farm animals is successful only when it is done exactly during the estrus
phase. Very specially, in buffalo the detection of estrus isproblematic since it is a silent-heat animal in which there
is no outwardly manifested clear signs of estrus (Suthar and Dhami, 2010). Realizing this problem, there have been
several attempts to device methods to overcome the difficulties of estrus detection in buffaloes, but most of the
methods developed till date have not yet been successful and reliable. There is, thus, pertinent need to develop an
efficient estrus detection method in buffaloes in order to enhance the success rate of conception through artificial
insemination.