The minimal inhibitory
concentration (MIC) was determined on 96 well culture plates
by a microdilution method using a microorganism suspension at a
density of 105 CFU.mL21 with Casein Soy Broth incubated for
24 hours at 37uC for bacteria, and Sabouraud Broth incubated for
72 hours at 25uC for yeasts. The cultures that did not present
growth were used to inoculate plates of solid medium (Muller
Hinton Agar and Sabouraud Agar) in order to determine the
minimum bactericidal/fungicidal concentration (MBC/MFC).
Proper blanks were assayed simultaneously and samples were
tested in triplicate. Technical data have been described previously
[25,26].