2.5. Serum and plasma analysis
The detection of endotoxin in plasma was performed using the
quantitative chromogenic tachypleus amebocyte lysate method with
endotoxin test kits (Chinese Horseshoe Crab Reagent Manufactory,
Co., Ltd., China). And the measurement of diamine oxidase in plasma
was performed using enzymatic kinetic spectrophotometry method
with diamine oxidase test kits (Nanjing Jian Cheng Technology, Co.,
Ltd., China). The enzymatic kinetic spectrophotometry method with a
D-lactate test kit (Genmed Scientifics Inc. USA) was used to analyze
the concentrations of D-lactate in serum.