Par-simony analyses from the ITS region in nine Chilean Batrachiumand two European samples – one of R. trichophyllus and otherof R. aquatilis from GenBank – were performed using PAUP ver-sion 4.0b4a for Macintosh (Swofford, 2000) by heuristic searches.We used starting trees obtained by simple stepwise additionsequences, with one tree held at each step and the TBRbranchswapping algorithm “MuLTrees” option in effect, accelerated trans-formation (ACCTRAN), branches of zero length collapsed andtopological constraints not enforced.