Bacterial isolates, antimicrobial susceptibility testing, and detection of ESBL.
Bacteria recovered from clinical specimens were identified by standard biochemical
methods. The production of ESBL was tested for all E. coli and K. pneumoniae
isolates by using the combination disk method based on the inhibitory
effect of clavulanic acid according to the Clinical and Laboratory Standards
Institute (CLSI) criteria (13). Consecutive nonduplicate clinical isolates of
ESBL-producing E. coli and ESBL-producing K. pneumoniae were collected for
further investigation. The MIC50 and MIC90 values of six antimicrobial agents
(ceftazidime, cefotaxime, ceftriaxone, imipenem, ertapenem, and meropenem)
Bacterial isolates, antimicrobial susceptibility testing, and detection of ESBL.Bacteria recovered from clinical specimens were identified by standard biochemicalmethods. The production of ESBL was tested for all E. coli and K. pneumoniaeisolates by using the combination disk method based on the inhibitoryeffect of clavulanic acid according to the Clinical and Laboratory StandardsInstitute (CLSI) criteria (13). Consecutive nonduplicate clinical isolates ofESBL-producing E. coli and ESBL-producing K. pneumoniae were collected forfurther investigation. The MIC50 and MIC90 values of six antimicrobial agents(ceftazidime, cefotaxime, ceftriaxone, imipenem, ertapenem, and meropenem)
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