and enzyme systems from the established consortium with different
reaction specificities and their synergistic mechanism are thus
of great interest for biotechnological research and application. A
metagenomic library has been constructed and the cloning of genes
encoding the lignocellulolytic enzyme systems from the microbial
consortium is in progress. Based on the amino acid sequences of the
polypeptides deduced from proteomic analysis, this thus allows
direct cloning of the composite cellulolytic and hemicellulolytic
enzymes from the metagenome for further study on their synergism
in degradation of lignocellulosic substrates.