as described with slight modifications, or by using the DNeasy plant mini kit (Qiagen) after breaking the cells in Retsch MM400 Mixer Mill using 5 mm metal beads for several rounds of 40 s, 25 Hz or grinding by mortar and pestle in the presence of liquid nitrogen. PCR reactions were performed using the following DNA polymerases: Go-Taq (Promega), Ex-Taq (TAKARA) and PrimeStar HS (TAKARA) using the primers described in Table 1