For enzyme activities in exocarp or mesocarp,
five grams of
fresh tissue were homogenized in 25 mL of acetone at 4 C,
filtered,
and washed repeatedly with acetone. The
filtrate was suspended in
25 mL of 20 mM phosphate,1 M NaCl, pH 7.0 buffer and then stirred
for 35 h. The extract was centrifuged at 8000
g for 30 min at 4 C.
The supernatant was the raw enzymatic extract