In the first part of the thesis, we present an OR gate based on monoclonal antibodies for
the simultaneous detection of multiple toxins in a single tube. To further simplify the
operating procedure, the Boolean rule of simplification was used to guide the selection of a
marker toxin among the natural toxin profiles. Furthermore, we developed a cellular logic
circuit for deciphering the toxin profiles produced by B. cereus, using readout techniques
based on pore formation on the cell membrane. This new assay enabled the simultaneous
detection of seven biomarkers in pathogenic strains from various sources. Lastly, a cellular
logic system capable of combinatorial and sequential logic operations based on bacterial
protein-triggered cytotoxicity was constructed. Advanced devices such as a keypad lock,
half-adder and several basic Boolean properties were demonstrated on the cells.