Lipid blood tests and FA analysis. Serum concentrations of total
cholesterol (TC), HDL cholesterol, and TGs in feed-deprived mice were
quantified with commercially available kits from Instrumentation
Laboratories on a clinical chemistry analyzer IL650 (Instrumentation
Laboratories). Total lipids (TLs) were extracted from 200 mg ground
diets, 150 mg liver, 200 mg brain, and 100 mg muscle samples by using
chloroform/methanol (2:1 vol:vol) containing 0.01% BHT as an
antioxidant. FAs were analyzed by GC-Flame Ionization Detector
following the method described previously (38).