: (1) Our AD model was based on the intraperitoneal injection of D-gal combined with the injection of IBO into the basal nuclei. This type of AD model rat mimics certain symptoms of AD but does not completely recapitulate human AD. We based our AD model on several previous studies [18-20]. (2) We tested the development of AD in rats using only a Y-maze test, and analysing some a biochemical indicator may be better.