The most potent strain was identified using biochemical tests as Bacillus sp. N-2. Production of PHB was
carried out in limitation of nitrogen source using a minimal salt medium (MSM) supplemented with an excess of
glucose as sole carbon source. PHB was accumulated in relation to cell dry weight about 20% (PHB/CDW). The
obtained biopolymer was purified and analyzed using NMR, FT-IR, TGA and DSC thus; it was highly pure and
identified as PHB. Optimization of PHB production from cheap sources appears to be a realistic goal in the
future for reducing the costs and obtaining high yield.