The accuracy of the method was expressed as recovery, which is determined by analyzing of blank samples spiked with different concentrations of MG and LMG. The mean recoveries were in the range of 70.2e84.8% for MG, 72.1e85.6% for LMG, and 70.3e85.7% for MG þ LMG. The relative standard deviations were less than 9.9% and 11.5% for repeatability and within-laboratory reproducibility, respectively (Table 1). The results of stability test showed that the degradation of MG and LMG in trout muscle was less than 5.5% after 8 weeks of frozen storage at e20 C (data not shown).