Currently, a combination of pectinases, cellulases and hemicellulases as macerating enzymes has been used in
the extraction and clarification of fruit and vegetable juices (Galante et al., 1998; Grassin and Fauquembergue, 1996). The efficiency of biomass conversion to ethanol depends upon the ability of the microorganism used in the process to utilize the diverse carbon sources and amount of fraction present in biomass (Prasad, 2007). The objectives of this study were to investigate the influence of different temperatures, shaking hours and water content on bioethanol production by using rotten banana mixture (pulp and peel). In addition, to investigate the proper yeast concentration and enzymes for fermentation from banana mixture (skin and pulp) and to know the standard properties (viscosity, pH and metal content) of bioethanol for the use in petrol engine