This study was carried out in the farm and laboratories of the Department of Breeding
& Genetic, Sugar Crops Res. Inst., Agriculture Res. Center, Giza, Egypt and Genetics dept.,
faculty of Agric., Ain Shams University during 2009/2010 and 2010/2011 seasons. The aims
of this study were to evaluate 26 sugarcane clones for their sugar content and detect some
markers associated with sugar content using DNA-based PCR techniques to assist selection of
high sugar content promising lines at a very early stage of the sugarcane breeding program.
RAPD, SSR, ISSR and R-ISSR techniques were used for detecting markers associated to
sugar content. The performances of studied clones revealed that RAPD, SSR, ISSR and RSSR
techniques are useful as marker assisted selection for sugar content in sugarcane clones.
62 positive and 35 negative markers were obtained from used techniques, which could use as
marker assisted selection for sugar content. The study revealed that R-ISSR technique was
more efficiency in this case.