by Carelli and Echeverrigaray (2002). They
obtained multiple rates of 30.3 shoots per explant
of Rosa hybrida after 180 days on medium
containing salt formulation of Quoirin and
Lepoivre. This basal medium was supplemented
with 0.5 mg/l NAA and 3 mg/l BAP. However the
difference in results may be due to difference in
salt formulation of medium and cultivars tested.
The medium used in our studies and those of
Davies (1980).
During investigation, it was also observed
that 1.5 mg/l of BAP was best for shoot whereas
lower (0.5-1.0 mg/l) and higher concentrations
(2.0-2.5 mg/l) inhibited it. This was in accordance
with the study conducted by Kim et al. (2003) in
which they reported that lower concentrations of
BAP (1.0 – 1.5 mg/l) stimulated the bud growth in
six rose cultivars (Rosa hybrida L. cvs. “Sequoia
Ruby”, “Play boy”) but higher concentrations of
BAP (2.0-4.0 mg/l) inhibited shoot proliferation.
In summary, results from present
investigation manifest that micropropagation in
Rosa indica can be achieved from nodal explants
under physical and chemical conditions. However
the main focus of this study was to actually see the
reproducibility of previously reported protocols to
roses. To be useful at a commercial level,
micropropagation studies need further