f the rapid method with the inhibition index based on four
different determinations during the first 2 h with the usual
method. The agreement between the two methods was generally good, although some values are divergent.
Multiple determinations of the inhibition index of two urine
samples were performed and, as is evident from Table 1, the
inhibition index for the two urine samples ranged from 0.63
to 0.66 and 0.66 to 0.68, respectively. This corresponds to a
coefficient of variation of less than 1.5% in both series.
In Figure 3 we compare radioactivity measured after centrifugation or after Millipore filtration. There was a good
correspondence (r = 0.89).
Addition of 100 tL of 0.2 or 0.4 mmol/L sodium oxalate or
of 5 or 10 mmol/L calcium chloride did not measurably increase crystal growth rate, nor was there any notable effect on
the inhibition when magnesium chloride was added to give
final concentrations as high as 60 zmol/L.
The effects of sodium pyrophosphate, chrondroitin sulfate,
and citrate are shown in Figure 4. Sodium pyrophosphate in
a final concentration of 19 moVL completely inhibited crystal
growth.
Addition of HC1 to a final concentration of 0.19 to 1.9
mmol/L did not affect the crystal growth, whereas 9.4 mmol/L
(7) stopped it.
Storage of urine at room temperature resulted in a slight
decrease in the inhibition index, so slight as to be negligible
during the first 24-32 h. When the urine was stored frozen, the
inhibition index remained unchanged for several weeks.
Bacterial contamination significantly increased values for
the inhibition index.
Figure 5 illustrates the cumulative frequency distribution
curves of inhibition indices for normal men and men who are
stone formers. The mean inhibition indices were 0.56 (SD 0.06)
and 0.54 (SD 0.08), respectively; the difference is statistically
significant (p
f the rapid method with the inhibition index based on fourdifferent determinations during the first 2 h with the usualmethod. The agreement between the two methods was generally good, although some values are divergent.Multiple determinations of the inhibition index of two urinesamples were performed and, as is evident from Table 1, theinhibition index for the two urine samples ranged from 0.63to 0.66 and 0.66 to 0.68, respectively. This corresponds to acoefficient of variation of less than 1.5% in both series.In Figure 3 we compare radioactivity measured after centrifugation or after Millipore filtration. There was a goodcorrespondence (r = 0.89).Addition of 100 tL of 0.2 or 0.4 mmol/L sodium oxalate orof 5 or 10 mmol/L calcium chloride did not measurably increase crystal growth rate, nor was there any notable effect onthe inhibition when magnesium chloride was added to givefinal concentrations as high as 60 zmol/L.The effects of sodium pyrophosphate, chrondroitin sulfate,and citrate are shown in Figure 4. Sodium pyrophosphate ina final concentration of 19 moVL completely inhibited crystalgrowth.Addition of HC1 to a final concentration of 0.19 to 1.9mmol/L did not affect the crystal growth, whereas 9.4 mmol/L(7) stopped it.Storage of urine at room temperature resulted in a slightdecrease in the inhibition index, so slight as to be negligibleduring the first 24-32 h. When the urine was stored frozen, theinhibition index remained unchanged for several weeks.Bacterial contamination significantly increased values forthe inhibition index.Figure 5 illustrates the cumulative frequency distributioncurves of inhibition indices for normal men and men who arestone formers. The mean inhibition indices were 0.56 (SD 0.06)and 0.54 (SD 0.08), respectively; the difference is statisticallysignificant (p <0.05).
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