This study reveals that Hyperspectral Chemical Imaging system can be used to determine the adulteration of Khao Dowk Mali 105 (KDML 105) with Pathum Thani 1 (PTT 1), Chai Nat 1 (CNT 1) and Pitsanulok 2 (PSL2) by partial least square-discriminant analysis (PLS-DA) with two spectra pretreatment methods are very clearly