Propagation of Eulophia graminea Lindl. was conducted under in vitro culture. Seeds were cultured
on agar VW medium (Vacin and Went, 1949). After 2 months, shoots were transferred to agar VW medium
supplemented with various types of cytokinins (Thidiazuron, N6
-benzyladenine and N6
-furfuryladenine) and auxins
(indole-3-acetic acid, α-naphthaleneacetic acid and indole-3- butyric acid) at the concentrations of 5 and 1.0 mg/l
for 3 weeks compared with non-treated. The results showed growth and development of in vitro shoots were not
significant differences between the treatments in both cytokinins and auxins experiments. After acclimatized seedlings
with various growing media (coconuts husk chips, coconut husk chips: broken charcoals, coconut husk chips:
broken bricks, coconut husk chips: foams and sphagnum moss) for 3 weeks. The results were shown coconut husk
chips could promote seedlings growth and development with the maximum of new root numbers at 11.27 roots.
Keywords: tissue culture, orchid, plant growth regulators