preparation of pectin from durian rind
The extraction method was modified from the combination methods described by McCready(1970)and Hokputsa et al. (2004).The durian rind (solid-liquid ratio:1:9,w/v) were gently stirred in a mind acid aqueous solution adjusted to pH 2 with 1M HCI.Then the solution was extracted at 90 for 4hr.The resulting slurries were filtered through cheese cloth and allowed to cool to room temperature(25C).Acifiied ethanol(4%HCI in 95%EtOH) was added in the ratio 1:4 (v/v) and kept for 1 hr. The mixture was centrifuged at 3000 rpm for 15min in a Bench Top Centrifuge(Kubota 5100,Fujioka,Japan)