The experiment consisted of cultivating shrimp in different
concentrations of bioflocs, expressed as TSS in water. Three different
concentrations of TSS were studied (independent variable):
TSS up to 200 mg L−1 (T200); TSS between 400 and 600 mg L−1
(T400–600); TSS between 800 and 1000 mg L−1 (T800–1000). Each
tank was randomly assigned to a one-factor experimental design
with four replicates per treatment level. All of the tanks were
stocked with shrimp from the matrix tank. During the stocking
process, a selection based on weight was performed to reduce the
variability in animal size and the largest and smallest shrimp were
removed. In total, 390 juveniles of L. vannamei with an average
weight of 6.8 g were stocked in each tank, representing a stocking
density of 459 shrimp m−3 (390 shrimp m−2).