B. subtilis D10 showed the strongest antifungal activity followed by E. coli D2 producing 40 and 30 mm inhibition zone,
respectively against A. niger (Fig. 7). B. subtilis (D10) and E. coli (D2) showed inhibitory actions against the three tested
microbes. These results were supported by several studies which indicated the efficient antimicrobial activity of soil bacteria specially the Bacilli. In this connection, Aunpad and NaBangchang (2007) concluded that Bacillus pumilus isolated from the soil of Thailand produced new antimicrobial peptide with broad spectrum antibacterial activity including MRSA. The compound has potential for use as an alternative antibac terial agent for the treatment of infection with MRSA. Furthermore, the antimicrobial compound subtilin produced by B. subtilis was found to be effective against the commonly occurring gram positive S. aureus and Gram negative E. coli(Beima et al., 2002; Dhanapathi et al., 2008).