Biosensors: Biosensors are defined as indicators of biological compound that can be as simple as temperature sensitive paints. biosensor technology to food testing offers several attractive features. Many of the system are portable and hence can be used for field testing or on the spot analysis and are rapid test which are capable of testing multiple analysis simultaneously. Biosensing methods for pathogen detection are centered on four basic physiological or genetic properties of microorganisms. Many of today’s popular commercially available rapid methods use culture-based methods coupled with automated or semi-automated nucleic acids, antibody, or substrate utilization-based methods to obtain results in 24-72 h. Interestingly, many of the modern-day biosensor-based methods are developed utilizing one of the above four principles or combinations of some sort. However, antibody-based methods are the most popular because of their versatility, convenience and relative ease in interpretation of the data. It is interesting to note that a majority of biosensors use antibody for capture and detection of the target analyte