3. Results and Discussion
The polymorphism of CLPG gene was detected in 426 bp PCR product by restriction endonuclease FaqI
(Fig 2 a). The FaqI digestion of the PCR products produced digestion fragments of 395 bp and 31 bp for mutant allele G and 278 bp, 117 bp and 31 bp for wild allele A (Fig 2 b). In the present study, there was no
difference between digestion patterns and all sampled animals displayed AA genotype. As such, three 278,
117 and 31 bp amplified fragments from enzyme digestion were observed for all animals, indicating that the
total population of sheep was monomorphic for CLPG gene.