Experimental procedure for hydrodistillation and Soxhlet
extraction
Pine bark essential oil was separated by hydrodistillation (HD)
in a Schilcher apparatus, following the AOAC 962.17 method [24],
with a 33:1 (v/w) solvent-to-solid ratio. Soxhlet extraction was performed
using ethanol and a solvent-to-solid ratio of 50:1 (v/w).
Both systems were kept under reflux for 120 min at the solvent boiling
point (triplicate assays). EtOH was removed from the extracts
using a rotary evaporator with vacuum control. The pine essential
oil and Soxhlet extracts were kept at 255 K until further analysis