Immunonephelometric assay for alpha-1-antitrypsin (AAT) and PCR-reverse hybridization for Z and S alíele were first-line, and isoelectric focusing and DNA sequencing (ABI Prism BigDye) were reflex tests.
Immunonephelometric assay for alpha-1-antitrypsin (AAT) and PCR-reverse hybridization for Z and S alíele were first-line, and isoelectricfocusing and DNA sequencing (ABI Prism BigDye) were reflex tests.