Mimusops elengi, L. leaf was collected from Thiagarajar College
campus, Madurai where the work has being carried out. The silver
nitrate (AgNO3) was used as the starting materials obtained from
Sigma–Aldrich chemicals, USA. Fresh leaf of Mimusops elengi, L. was
washed repeatedly with milli-Q deionized water (Millipore Water
Unit, Bangalore, India). 5 g of leaf was finely cut and boiled with
100 ml of milli-Q deionized water for 5 min to get the extract. The
crude extract thus developed, was undergone a filtration method
by using Whatman No.1 filter paper. To optimize this reaction, the
following method was adopted: 100 ml aqueous filtrate of Mimusops
elengi, L. extract has being taken into 250 ml Erlenmeyer flask.
Then the extract was mixed with AgNO3, to make the final volume
concentration of 1 mM aqueous solution. This reaction mixture was
kept uninterrupted until its dark brown colour change was arisen.