The technique will be demonstrated first by the instructor. Observe all steps
carefully. Then carry out the inoculations as shown in all diagram.
Technique Source Target
A: Broth transfer S. aureus (NB broth) NB broth
B: Agar slants as sources of inoculum M. luteus (NA slant) NB broth
C: Inoculation of an agar slant S. aureus (NB broth) NA slant
D: Colony selection B. subtilis (NA plate) NB broth
E: Pour plate technique M. luteus+ S. aureus
(NB broth)
NA (tube) to
NA plate
F: Streak plate technique M. luteus+ S. aureus
(NB broth)
NA plat