Identification and taxonomy of actinobacteria are very important for the research of new compounds, providing information about the relations between organisms and about their potential secondary metabolites.9 An important method for identification of actinobacteria is the sequencing of the 16S region of their ribosomal DNA (rDNA). This region is conserved and present in all bacteria.10 Comparison of sequences from unidentified isolates with those already known allows the construction of phylogenetic trees and identification of organisms.11 The aim of this work was to evaluate inhibitory activity of actinobacteria against melanized fungi and identify the actinobacterial isolates to the species level.