The multi-screening assays to establish optimal
immobilization conditions require the expense of a
large amount of carrier, enzyme and reagents (buffers,
washing solutions, etc.). In order to minimize the
consumption of these components, and especially of
the most valuable (the enzyme and, in many cases, the
support), we present in this work a simple and
inexpensive methodology based on the use of microcentrifuge
filter tubes, which allow to carry out the
screening experiments in a scale of milligrams of
carrier and microliters of enzyme with great reproducibility.
Such methodology is also applicable to
assess the apparent activity of the resulting immobilized
biocatalysts, as well as to simulate their
operational stability under batch conditions