Explants derived from different parts of the mother plants, after initiate growth on
the callus induction medium, can be developed into plantlets. However, not all types of
explants are favorable for root stimulation. Shen et al. [1995] found that when both
clustered shoots (with 2–5 shoots) and a single shoot tip were used as explants to be
subcultured on 1/2 MS medium with 0.1 ml·L-1 NAA, a higher rooting percentage
(78.9%) was received from clustered shoots in comparison to that from a single shoot
tip, which only gave 25.7% rooting. Fortes et al. [1997] and Gebler [2005] illustrated
similar results. Fortes et al. [1997] studied the influence of young shoot number on
asparagus rooting. It was reported that four-shoot explants rooted better than those with
one, two or three shoots. Longer roots were also observed from the explant with four
shoots. Gebler [2005] stated that none of the single shoot explant could develop storage
roots.