For detection of E. coli O157:H7, 2 μl of the enriched broth fromboth
PED and the stomacher bagwas directly used for the real-time qPCR in a
25 μl reaction volume using the Taqman® E. coli O157:H7 detection kit
(Kit # 4307514, Applied Biosystems, Foster City, CA) and amplified
using the Applied Biosystems StepOne thermocycler.