Sampling and isolation of bacterium
During spring and summer 2011, sampling from 10 rainbow trout farms in Chaharmahal-va-
Bakhtiari Province was done. Infected fishes or suspected to diseases were randomly sampled
from selected farms and then from some organs such as liver and intestine, Sterile swabs were streaked on TSA
plate and plates were transferred to the lab beside the ice.
Isolation of pathogens and biochemical analysis
Plates transferred to the laboratory were incubated at 25°C for 48 h for growing the colonies. Single colonies