a b s t r a c t
The present study was aimed to investigate the culture influence on pectin lyase production
potential of fungal strain Aspergillus oryzae. The enzyme profile of A. oryzae showed
highest activity of pectin lyase after 3rd day of incubation on lemon peel waste under solid
state fermentation conditions. To induce the pectin lyase synthesis capability of A. oryzae
at optimal level various culture variables including physical and nutritional parameters
were optimized by adopting classical optimization technique. Therefore, through
fermentation process optimization the production of pectin lyase was substantially
induced up to the level of 875 U/mL, when fermentation medium of lemon peel waste
inoculated with 5 mL spore suspension of A. oryzae. The optimal fermentation conditions
for maximum pectin lyase yield were as: optimum pH 5, 70% moisture level and incubated
at 40 C in addition with 1% sterile glucose solution as readily available carbon source and
0.2% yeast extract as an inexpensive nitrogen supplement (1%). The results obtained in
current investigation so far demonstrated that culture conditions have great influence on
the pectin lyase production potential of A. oryzae.
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