Most of the antibiotic producers used today are the soil microbes. Fungal strains and streptomyces members are extensively used in industrial antibiotic production. Bacteria are also reported for their antibiotic production. Bacillus species being the predominant soil bacteriabecause of their resistant endospore formation and production of vital antibiotics like bacitracin etc. have always been found inhibiting the growth of the other organisms. It is advisable to screen antibiotic producing bacteria as they are easy to isolate, culture, maintain and to improve their strains. Keeping in view the abovementioned points and antimicrobial activity of soil isolates, the present study was aimed to isolate antibiotics producing strains and screening of isolates against test organisms.