number of shoots per explant in most PGRs combinations
compared to the other periods (0, 20, or 30 days). However,
the highest frequency of shoot regeneration as well as the
mean number of shoots per explant were obtained using a
medium containing a combination of 1 mg L1 of BA and
2 mg L1 of NAA under continuous light conditions without
any initial dark period. Thus, this medium composition and
culture conditions were used in further experiments.