represents 16 adults of L reevesii collected between 2007 and
2008 from each of 28 sampling localities almost covering the species’
distribution range from Chinese mainland (Guangdong and
Guangxi) to Hainan (the largest island second to Taiwan in China)
and Vietnam (Fig. 1). During our collecting trip to the coastal region
of Vietnam and Guangxi (a province of China), we did not find
any L. reevesii in the north of Vietnam and in the west of Guangxi,
close to Vietnam. This is the region where the microcontinental
blocks of Southeast Asia and the megacontinent of Asia contacted
(Fig. 1), currently having no habitat suitable for L. reevesii.
The most distal 15 mm of the tail tip of each lizard was excised
using a sterilized scalpel. Individual lizards were released at their
site of capture after tissue sampling. Tissue samples were preserved
in 95% ethanol before they were deposited at Hangzhou
Normal University and assigned voucher numbers identified by
locality-haplotype numbers. Total DNA was extracted using standard
phenol–chloroform methods (Sambrook et al., 1989), and
stored at 80 C until ready for use.