As an important indicator measuring enterprise management level, psychological ecology level of enterprise organization is an important basis for behavior prediction and management decision. Enterprise organization is made up of individual. The combination of individual psychological state decides the psychological ecology of enterprise organization. The work experience of individuals is mainly exhibited by job satisfaction. To carry out evaluation on the psychological ecology of organization, this paper constructs the dimensional system of job satisfaction degree. On the basis of this, a corresponding questionnaire and testing methods of reliability and validity of the questionnaire are designed. The dimensional objective empowerment methods and entropy evaluation methods of evaluating organization psychological ecology level are presented. Finally, the author developed investigation and evaluation system based on internet environment and a case study about an enterprise is implemented. The results show that this measurement method can exhibit the status of dimensions of organization psychological ecology according to the importance of dimensions, to provide the necessary decision-making basis for managers.