Synbiotics consisted of B. breve M-16V (Morinaga Milk Industry Co, Ltd., Tokyo, Japan) at a dose of 1.3 × 109 cfu/100 ml and a mixture of 90% scGOS and 10% lcFOS (Immunofortis®), 0.8 g/100 ml (22). Formula was given on demand. The product was stable for at least 18 months when stored at room temperature (20–25°C).