2.2.7. Data analysis and statistics
Solution and scaffold properties (weight loss and fiber diameter)are presented as the mean value±experimental standard deviation of the mean.
The cellular viability of 3T3 fibroblasts is presented as the number of cells±combined standard uncertainty.
Fiber diameters were measured on the SEM images by using the ImageJ software.
The weight loss study and the cell culture experiments were performed in triplicate for each different crosslinking procedure.
Images acquired by confocal microscopy were analyzed using also the ImageJ software to automatically count the number of nuclei and cell-area covered per imaged field, to manually score the number of mitotic cells per field, and for qualitative assessment of abundance of filopodia and stress fibers.
Both values of cell-areadetermined by confocal images and cell proliferation were compared using a t-test, with p-valuesb0.05 considered statistically significant.