2.4.3. Catalase activity (CAT)
The assay is based on the determination of the rate of hydrogen peroxide (E.
Merck) decomposition at 240 nm [32]. The reaction was conducted at a constant
temperature (30
◦
C) for 1 min. The enzymatic activity was expressed as catalase
units per mg of protein. One unit of catalase decomposed 1 mol of H2O2per min
at pH 7.4 and 30
◦
C