A. Reflux 1 g of the sample , in powder , with 30 ml of ethanol for 15 minutes, and filter. To 2 ml of the filtrate, add a few drops of amnanium malybdate TS : a bright yellow precipitate is produced.
B. To 2 ml of the filtrate obtained from test A, add a greenish brown colour is produced.
C. To 2 ml of the filtrate obtained from test A, add a few drops of ninhydrin TS and warm on a water-bath for a few minutes: a reddish purple colour is produced.
D. Moisten 1 g of the sample, in powder, with 0.5 ml of strong ammonia solution, add 5 ml of chloroform, occasionally shaking for 10 minutes, add filter. Evaporate the filtrate until dryness and dissolve the residue in1 ml of ethanol, and add a few drops of modified Dragendorff TS 2: an orange precipitate is produced.