disposable, cost effective screen-printing biosensor for ALT measurement. This biosensor will be useful for clinical or point-of-care quantification of ALT in human serum. The experimental approach and results are given. Furthermore, the experimental quantifications of ALT in human serum of this biosensor prototype are compared to the “gold standard” spectrophotometric assays of a clinical laboratory, and the results are in excellent agreement. Thus, this single use, disposable, biosensor provides a simple detection method for quantification of ALT in human serum as an excellent technology for clinical and point of care ALT detection.