Four varieties of lima beans (Phaseolus lunatus L. WALP) seeds with
accession number NSWP100, NSWP98, NSWP47 and NSWP96 were
obtained from the Institute of Agricultural Research and Training
(IAR&T), Ibadan, Nigeria. The cleaned lima beans were cracked with
hammer mill, soaked in water for 3 h and dehulled. Hull was dried at
(60 ± 2 °C) for 24 h, milled and sieved to give 40 mm mesh size flour
and kept in airtight plastic containers at 4 °C prior to use. Chemicals
used were of analytical grade.