Abstract
A method is described for the estimation of pantothenic acid in milk and infant formula based on deproteination of sample and
direct analysis by reversed-phase HPLC with a low pH phosphate:acetonitrile eluent and multiwavelength UV detection. Several
LC columns were evaluated and a C8 chemistry selected for routine application. Comparative data are provided against compendial microbial and GLC procedures. Based on the low content of bound, endogenous pantothenate in milk, the method provides a reliable measure of total vitamin B5 for compliance control during infant formula production. # 2000 Elsevier Science Ltd. All rights reserved