A common
approach is to segregate isolates on the basis of colony morphology and identify
genus and species by rRNA gene-sequencing from only select morphotypes.
A drawback to sequencing methods is the cost of sequencing which, while decreasing,
is still prohibitive for large-scale ecological studies.
In addition to the rRNA gene sequences themselves, the spacer regions between
the rRNA genes in yeast (Montrocher, et al. 1998; Egli and Henick-Kling 2001;
Belloch, et al. 2002) and bacteria (Le Jeune and Lonvaud-Funel 1997) have been
used to further differentiate both yeast and bacteria in wine, primarily assisting in
subspecies differentiation.
A commonapproach is to segregate isolates on the basis of colony morphology and identifygenus and species by rRNA gene-sequencing from only select morphotypes.A drawback to sequencing methods is the cost of sequencing which, while decreasing,is still prohibitive for large-scale ecological studies.In addition to the rRNA gene sequences themselves, the spacer regions betweenthe rRNA genes in yeast (Montrocher, et al. 1998; Egli and Henick-Kling 2001;Belloch, et al. 2002) and bacteria (Le Jeune and Lonvaud-Funel 1997) have beenused to further differentiate both yeast and bacteria in wine, primarily assisting insubspecies differentiation.
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