In a typical experiment, 1.4 g of extracted and purified microalgal lipids (95 wt% SL purity) was mixed with 13.3 mL of solvent (hexane or t-butanol) (10 mL solvent/g SLs), 0.93 g of R. oryzae fungus (0.7 g of R. oryzae/g SLs) and 2.1 mL of methanol (methanol/SL molar ratio11:1).