Seed treatment was done using a suspension of B. subtilis (strain
160) at 1 108 cells/mL, obtained from a 60 h PDB culture by infiltration
into the seeds using a vacuum. The control seeds were infiltrated
with an equal volume of PDB without the bacteria, diluted in
water at the same proportion as the suspension of B. subtilis. The
sensitivity of B. subtilis to the insecticide Poncho 600 FS was
tested as follows: 5 seeds treated with insecticide and B. subtilis
were sown in plates with PDA and incubated at 28 C for 48 h.
Development of the bacteria was observed.